Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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R&D Systems™ Human IL-12 p70 Quantikine ELISA Kit
Human IL-12 p70 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Human IL-12p70 |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 3592 |
| Sample Type | Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum,Urine |
| Sample Volume | Cell Culture Supernates (200uL),Serum (200uL),EDTA Plasma (200uL),Heparin Plasma (200uL),Citrate Plasma (200uL),Urine (200uL) |
| Synonym | CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12 A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35 |
| Assay Sensitivity | 5pg/mL |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 7.80 - 500pg/mL |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules |
Invitrogen™ Human Galectin-9 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Lgals9 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 36 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 36.86 to 9,000 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | 36 kDa beta-galactoside-binding lectin; AA407335; AI194909; AI265545; Ecalectin; Gal9; gal-9; galectin 9; Galectin9; galectin-9; HOM HD 21; HUAT; Lectin galactose binding soluble 9 (Galectin-9); lectin, galactose binding, soluble 5; lectin, galactose binding, soluble 9; Lectin, galactose binding, soluble 9 (Galectin-9); lectin, galactoside binding soluble 9; lectin, galactoside-binding, soluble, 9; LGALS35; Lgals5; LGALS9; LGALS9A; Tumor antigen HOM-HD-21; UAT; Urate transporter/channel; urate transporter/channel protein |
| Regulatory Status | RUO |
| Target | Galectin 9 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3965 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human GFAP ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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R&D Systems™ Human Lipocalin-2/NGAL Quantikine ELISA Kit
Human Lipocalin-2/NGAL Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Human Lipocalin-2 |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 3934 |
| Sample Type | Cell Culture Supernatant,Plasma (Heparin),Saliva,Serum,Urine |
| Sample Volume | Cell Culture Supernates (50uL),Serum (50uL),Heparin Plasma (50uL),Saliva (50uL),Urine (50uL) |
| Synonym | 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, lipocalin 2, lipocalin 2 (oncogene 24p3), Lipocalin2, Lipocalin-2, migration-stimulating factor inhibitor, MSFI, neutrophil gelatinase-associated lipocalin, NGAL, NGALlipocalin-2, Oncogene 24p3, p25, Siderocalin, Uterocalin |
| Assay Sensitivity | 0.04ng/mL |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 0.16 - 10ng/mL |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules. |
Invitrogen™ Thyroxine (T4) Competitive ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Description | Thyroxine (T4) Competitive ELISA Kit |
| Interassay CV | 7% |
| Intraassay CV | 3 |
| Total Assay Time | 1 hrs 30 mins |
| Sample Volume | Fecal Extract 10 μL, Plasma 1 μL, Serum 1 μL, Supernatant 10 μL, Urine 2.5 μL |
| Assay Sensitivity | 0.29 ng/mL |
| Storage Requirements | -20°C |
| Research Discipline | Metabolism |
| Assay Range | 0.625 to 20 ng/mL |
| For Use With (Application) | ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti |
| Test Time | 1 hrs 20 mins |
| Detection Method | Fluorescence |
| Regulatory Status | RUO |
| Target | Thyroxine (T4) |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Fecal Extract,Plasma,Serum,Supernatant,Urine |
| Label or Dye | HRP |
Invitrogen™ Rat Growth Hormone ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ PKA (Protein Kinase A) Colorimetric Activity Kit
Assay PKA (Protein Kinase A) Activity in Cell Lysates, Tissue Extracts and Buffer Samples.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Assay Activity Kit |
| Content And Storage | 96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet. Store at –20°C. |
| For Use With (Equipment) | Microplate Reader |
| Target Kit Named | PKA (Protein Kinase A) |
Invitrogen™ Mouse S100A8/S100A9 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | S100A8, S100A9 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.65 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.65 to 150 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | 60B8Ag; AI323541; AW546964; B8Ag; BEE11; BEE22; Caga; Cagb; calgranulin A; calgranulin B; calgranulin-A; Calgranulin-B; calprotectin; calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; CGLA; CGLB; Chemotactic cytokine CP-10; chemotactic S100 protein; CP-10; Cystic fibrosis antigen; EGK |
| Regulatory Status | RUO |
| Target | Calprotectin |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 20201, 20202 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Superoxide Dismutase (SOD) Colorimetric Activity Kit
Assay Superoxide Dismutase (SOD) Activity in Serum, Plasma, Cells, Tissues and Erythrocyte Lysates.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Assay Activity Kit |
| Content And Storage | 96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet. Store at –20°C. |
| For Use With (Equipment) | Microplate Reader |
| Target Kit Named | Superoxide Dismutase (SOD) |
Invitrogen™ Human ALKP ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | ALPL |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 2 mIU/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 2 to 500 mIU/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | Akp2; Akp-2; Alkaline phosphatase 2; alkaline phosphatase 2, liver; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase precursor (EC 3.1.3.2); alkaline phosphatase tissue non-specific isoform; alkaline phosphatase tissue non-specific isoform/TNS-AP; alkaline phosphatase, biomineralization associated; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; Alpl; APTNAP; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney isozyme; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; PHOA; tissue-nonspecific alkaline phosphatase; tissue-nonspecific ALP; Tissue-nonspecific ALP alkaline phosphatase; TNAP; TNSALP; TNS-AP |
| Regulatory Status | RUO |
| Target | ALPL |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 249 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human IL-6 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
|---|---|
| Description | Human IL-6 ELISA Kit |
| Precision | Intra-assay CV <10%; inter-assay CV <10% |
Invitrogen™ Rat TNF alpha ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Rat |
|---|---|
| Conjugate | HRP |
| Interassay CV | 10% |
| Intraassay CV | 5% |
| Total Assay Time | 3 hr 10 min |
| Sample Volume | Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 11.0 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 39.1-2500 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | P16599 |
| Regulatory Status | RUO |
| Target | TNF alpha |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 24835 |
| Sample Type | Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | Cachectin, Tumor necrosis factor alpha, DIF |
Invitrogen™ Human GM-CSF ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Gene ID (Entrez) | 1437 |
| Interassay CV | 4.8% |
| Intraassay CV | 5.7 |
| Assay Sensitivity | <3 pg/mL |
| Assay Range | 7.8 to 500 pg/mL |
| For Use With (Application) | Colorimetric Detection |
Invitrogen™ Mouse IgG1 Uncoated ELISA Kit with Plates
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hr 30 min |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 50 μL |
| Assay Sensitivity | 3.13 ng/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 3.13-200 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 45 min |
| Regulatory Status | RUO |
| Target | Mouse IgG1 |
| Kit Contents | Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Immunoglobulin G1 |
Invitrogen™ Human IDO ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Ido1 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.81 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.819 to 200 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | EC 1.13.11.52; Ido; IDO1; IDO-1; Indo; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; indoleamine 2,3-dioxygenase 1; indoleamine 23-dioxygenase; Indoleamine-2; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase |
| Regulatory Status | RUO |
| Target | IDO |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3620 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |