Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (81,357)

Singleplex and Multiplex Bead Based Immunoassays (5,714)

ELISA Substrates and Reagents (2,155)

61 – 75 of 86,733 results

Invitrogen™ Mouse IL-12 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Interassay CV	10%
Intraassay CV	5%
Total Assay Time	3 hr 10 min
Sample Volume	Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	4.0 pg/mL
Storage Requirements	2-8°C
Assay Range	31.3-2000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P43431, P43432
Regulatory Status	RUO
Target	IL-12 p70
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16159, 16160
Sample Type	Serum,Supernatant
Label or Dye	Biotin
Synonym	CMLF, NKSF, Interleukin 12

Invitrogen™ Mouse IL-10 Instant ELISA™ Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	16153
Interassay CV	10.1%
Intraassay CV	6.7
Assay Sensitivity	5.28 pg/mL
Assay Range	39.1 to 2,500 pg/mL
For Use With (Application)	ELISA

Invitrogen™ Human Complement C5a ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Intraassay CV	3.6
Total Assay Time	4 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 5 μL, Supernatant 50 μL
Assay Sensitivity	0.005 ng/mL
Assay Range	0.078 to 5.0 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Regulatory Status	RUO
Target	Complement C5a
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	727
Sample Type	Plasma,Serum,Supernatant
Synonym	complement component 5, C5a, C5D, C5b, ECLZB, CPAMD4

Invitrogen™ Rat IL-6 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Rat
Conjugate	HRP
Interassay CV	10%
Intraassay CV	5
Gene Symbol	ILg6,Ifnb2
Total Assay Time	3 hrs 10 mins
Sample Volume	Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	12.0 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	31.3 to 2,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P20607
Regulatory Status	RUO
Target	IL-6
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	24498
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Interferon-beta 2, B-cell stimulatory factor 2,HGF

Invitrogen™ Mouse IFN gamma ProQuantum Immunoassay Kit

ELISA

Pricing & Availability
Specifications

Immunoassay Kit Format	Proximity-based Immunoassay
Target Species	Mouse
Conjugate	FAM™
Incubation Time	1 hr.
Gene Symbol	IFG; IFI
Total Assay Time	2 hrs
Sample Volume	Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity	<0.05 pg/mL
Storage Requirements	Frozen at -20°C
Assay Range	0.064 to 5,000 pg/mL
For Use With (Application)	Protein Quantitation
Test Time	30 mins
Detection Method	Taqman™ Real-time PCR
Accession Number	P01580
Regulatory Status	RUO
Target	IFN gamma
Kit Contents	Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment)	qPCR Instruments
Product Type	ProQuantum
Gene ID (Entrez)	15978
Sample Type	Plasma,Serum,Supernatant
Label or Dye	FAM™

Invitrogen™ Testosterone High Sensitivity Competitive ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Interassay CV	<10%
Intraassay CV	<6
Assay Sensitivity	2.97 pg/mL
Assay Range	7.8 to 1000 pg/mL

Invitrogen™ Human AMPK alpha-1,2 (Phospho) [pT172] ELISA Kit

ELISA

Pricing & Availability

Invitrogen™ Human IL-2 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	Il-1 A
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P60568
Regulatory Status	RUO
Target	IL-2
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3558
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-2

R&D Systems™ Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit

Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Target	CCL2/JE/MCP-1
Specificity	Natural and Recombinant Mouse MCP-1
Host Species	Mouse
Gene ID (Entrez)	6347
Gene Symbol	CCL2
Total Assay Time	4.5 hr.
Sample Volume	Cell Culture Supernates (50 uL),Tissue Lysates (50 uL),Tissue Culture Supernates (50 uL),Serum (50 uL),EDTA Plasma (50 uL),Heparin Plasma (50 uL)
Assay Sensitivity	0.666 pg/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	7.81 - 500 pg/mL
For Use With (Application)	ELISA

Invitrogen™ Mouse IL-6 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Interassay CV	6.7%
Intraassay CV	5.8
Gene Symbol	IL-6
Total Assay Time	3 hrs 30 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity	<3 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	7.8 to 500 pg/mL
For Use With (Application)	Colorimetric
Test Time	1 hrs 20 mins
Accession Number	P08505
Regulatory Status	RUO
Target	IL-6
Kit Contents	Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision	<3 pg/mL
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16193
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Human Tau (Phospho) [pT231] ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	4137
Interassay CV	7.8%
Intraassay CV	5.4
Assay Sensitivity	<1 U/mL
Assay Range	1.56 to 100 U/mL

Invitrogen™ Human Complement Factor H ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	CFH
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	2 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 500 ng/mL
Test Time	1 hrs 20 mins
Accession Number	adrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1 H; beta-1-H-globulin; Cfh; CFHL3; complement component factor H; complement factor H; complement factor H related protein 3A4/5G4; complement factor H, isoform b; complement inhibitory factor H; factor H; factor H-like 1; FH; FHL1; FHL1 antibody; H factor 1; H factor 1 (complement); H factor 2 (complement); HF; HF1; HF2; HGNC:4883; HUS; MGC88246; Mud-1; NOM; platelet complement factor H; protein beta-1-H; RP1-177P10.1; Sas1; Sas-1
Regulatory Status	RUO
Target	Complement Factor H
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3075
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Mouse TNF alpha ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Interassay CV	5.7%
Intraassay CV	6.5
Gene Symbol	DIF,TNF-a,TNF-alpha,TNFSF2,TNFalphaa,Tnfsf1a,Tnlg1f,Tnf
Total Assay Time	3 hrs 30 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	3.7 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	31.3 to 2,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P06804
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	21926
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Cachectin, Tumor necrosis factor alpha, DIF

Invitrogen™ Human VEGF-A ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	4.3%
Intraassay CV	6.2
Gene Symbol	MVCD1,VEGF,VPF
Total Assay Time	4 hrs 30 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	7.9 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	15.6 to 1,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P15692
Regulatory Status	RUO
Target	VEGF
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7422
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Vascular endothelial growth factor A, VPF

Invitrogen™ Mouse Granzyme B ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Intraassay CV	3.5%
Total Assay Time	3 hr 30 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	1.6 pg/mL
Assay Range	7.8-500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Granzyme B
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	14939
Sample Type	Plasma,Serum,Supernatant
Synonym	Granzyme 2, Fragmentin 2, Human lymphocyte protein

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Invitrogen™ Mouse IFN gamma ProQuantum Immunoassay Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Mouse IFN gamma ProQuantum Immunoassay Kit